Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mitch Paquette
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:13
Origin in AB, Forward primer
| Types | DnaRegion |
| Roles | primer Primer |
| Sequences | BBa_K187424_sequence (Version 1) |
Description
The pUC19 origin can be amplified from pAB or pBA using this primer in conjunction with the Origin AB reverse primer. A high copy origin can be produced with A and B ends compatible with the BioBytes assembly method. Origins cannot be cloned into pAB and pBA using the standard BioBytes method because the presence of two origins will destabilize the plasmid. Some thymine bases in the above sequence must be replaced with uracil for correct function. Actual sequence is as follows:5' AGGAAGUACGGUGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC 3'
This part will allow amplification of a high copy origin to allow proliferation of an artificial BioBytes plasmid. See BBF RFC#47 for information on the BioBytes method.
Notes
Primer was designed to produce the standard BioBytes A overhang. Spacing of uracils were experimentally optimized.Source
Synthetic| Sequence Annotation | Location | Component / Role(s) |
| should be uracil should be uracil | 7,7 12,12 | sequence_feature feature/misc sequence_feature feature/misc |