Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margaret Lie
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 04:26:40
iRFP670
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1878000_sequence (Version 1) |
Description
iRFP670 is a fluorescent protein that has an excitation wavelength of 643 nm and an emission wavelength of 670 nm. It utilizes billiverdin as a chromophore. The extinction coefficient is 114,000 M−1 cm−1, and the quantum yield is 11.1%. It is twice as bright as iRFP713 (formerly known as iRFP). The photobleaching half-life is 290 s and pKa is 4.0. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.Notes
The PstI site within the reporter has been removed. There are several BsaI sites within the protein so do not use the BsaI enzyme for Golden Gate DNA Assembly.Source
iRFP670 is a mutated form of the Rhodopseudomonas palustris bacterial phytochrome photoreceptor.Shcherbakova, D. M. & Verkhusha, V. V. Near-infrared fluorescent proteins for multicolor in vivo imaging. 10, (2013).