| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_K1878001_sequence (Version 1)
|
Description
This fluorescent protein has an excitation wavelength of 690 nm and an emission wavelength of 713 nm. The extinction coefficient is 98000 M-1 cm-1. Its quantum yield is 6.3%. Its photostability half-life is 960 seconds. Its pKa is 4.5. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.
Notes
The PstI site and EcoRI site within the reporter has been removed. There is a Bbs1 site within the coding sequence so be wary if using that enzyme for Golden Gate DNA assembly.
Source
iRFP713 is a Rhodopseudomonas palustris bacterial phytochrome photoreceptor.
