BBa_K1879001

BBa_K1879001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1879001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Madeleine Stone
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 09:46:00

promoter + CBZ cleavage enzyme + terminator



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1879001_sequence (Version 1)

Description

This part contains the CBZ-cleavage enzyme with a promoter (BBa_J23118) and RBS upstream of the coding sequence, and terminator (BBa_J61048) downstream of the sequence to make a fully functioning BioBrick. This BioBrick is used in our biocontainment system to cleave the CBZ protecting group from leucine prior to incorporation of leucine into proteins. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.

Notes

The promoter and terminator were cloned into the vector containing the cleavage enzyme.

Source

The coding sequence of this CBZ cleavage enzyme came from a study by Patel et al. See Part:BBa_K1879000, which is just the enzyme coding sequence. The promoter (BBa_J23118) and terminator (BBa_J61048) came from the iGEM kit.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1879001/1