| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K1879002_sequence (Version 1)
|
Description
This part contains a mutated leucyl-tRNA synthetase (leuS) gene, and a terminator (BBa_J61048). The synthetase gene has been reengineered to contain the necessary mutations for the synthetase to use CBZ-leucine and not normal leucine when charging leucyl-tRNA. The catalytic site of the enzyme has been modified to be complementary to CBZ-leucine rather than leucine, and the editing site has been modified to be non complementary to CBZ-leucine while being complementary to normal leucine. This mutated leuS part is necessary for charging leucyl-tRNA synthetase with CBZ-leucine, thereby generating a dependency on CBZ-leucine for the purposes of containing the genetically engineered organism. The strength of expression of this part can be varied in the organism by adding a promoter and RBS of a desired strength. This allows for easy manipulation of this part by future teams to best serve their purposes.
Notes
The mutated leuS sequence was commercially synthesized and the terminator was in the iGEM kit.
Source
The original leuS gene originates in E. coli. The sequence for the mutated leuS gene was generated by our team using mutagenesis and docking software. The terminator in this part is from the iGEM kit (BBa_J61048).
