BD-CIB1

BBa_K1884001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1884001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chengrong Xie
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-15 07:40:18

Gal4BD-CIB1



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1884001_sequence (Version 1)

Description

CIB1, a basic helix-loop-helix (bHLH) protein, would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1

Notes

Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1. They interact in a blue light-specific manner in yeast and Arabidopsis cells, acting together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. The modules require no exogenous chromophore, trigger protein interactions on a subsecond time scale that are reversible within minutes and can be activated by two-photon microscopy, allowing potential use in vivo in whole organisms.

Source

GalBD+CIB1

Sequence Annotation Location Component / Role(s)
Gal4BD
CIB1
1,519
526,1533
feature/protein CDS
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1884001/1