Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1884001_sequence (Version 1)
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Description
CIB1, a basic helix-loop-helix (bHLH) protein, would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1
Notes
Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1. They interact in a blue light-specific manner in yeast and Arabidopsis cells, acting together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. The modules require no exogenous chromophore, trigger protein interactions on a subsecond time scale that are reversible within minutes and can be activated by two-photon microscopy, allowing potential use in vivo in whole organisms.
Source
GalBD+CIB1