AD-CRY2

BBa_K1884002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1884002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chengrong Xie
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-13 08:55:18

VP16AD-CRY2



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1884002_sequence (Version 1)

Description

Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact with CIB1. This part is a Gal4 DNA activating domain fused to N terminus of CRY2.

Notes

One powerful application of protein-interaction dimerizers(CRY2-CIB1) is to allow inducible control of a 'split' protein, in which two inactive fragments are brought together to reconstitute a functional protein activity. In principle, using this approach with optically controlled dimerizers would confer light-dependent activity to a diverse range of target proteins. We used the CRY2-CIB1 modules to reconstitute split versions of Gal4 transcription factor proteins. Based on the transient nature of the CRY-CIB interaction,the light pulses would allow precise, dose-dependent control of protein activity. We expressed in yeast the split Gal4 partners Gal4BD- CIB1 and Gal4AD-CRY2 together with upstream activating sequence under control of a galactose-inducible promoter. We incubated the yeast in the dark or subjected them to up to twenty blue-light pulses over 4 h. Immunoblot analysis of Snl1 revealed a strong dose dependence of protein expression in response to light.

Source

Part cloned from pRMH120 of Addgene

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1884002/1