Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 04:12:34
N-Luciferase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1891003_sequence (Version 1) |
Description
It encodes N-terminal of luciferase. When it meets C-terminal of luciferase, the N-terminal and C-terminal can become a complete luciferase. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of N-Luciferase. We use it as a part of reporter gene to detect whether it has taxol or its analogues.Notes
To get this part, we designed primers according to the gene sequence and then cloned it to our expression vector.Source
We get this gene from pCambia1300-N-Luciferase plasmid.| Sequence Annotation | Location | Component / Role(s) |
| N-Luciferase | 1,1248 | sequence_feature feature/misc |