BBa_K1896005

BBa_K1896005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 05:41:52

mSA2-His

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1896005_sequence (Version 1)

Description

Variant of [[Part:BBa_K1896000|Part:BBa_K1896000]] for use in scarless C-terminal protein fusions. This part contains a His-tag instead of a terminal AgeI restriction site.

Notes

This part is not compatible with the Freiburg assembly standard ([[Assembly_standard_25|RFC 25]]).

Source

Combination of streptavidin (Streptomyces avidinii) and rhizavidin (Rhizobium etli), engineered through homology modeling [1].

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GSTGS linker	1,15	CDS, feature/cds
mSA2	16,372	feature/cds, CDS
His-tag	373,390	CDS, feature/cds
stop	391,396	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896005/1

Member of these Collections

• iGEM Parts Registry