BBa_K1896008

BBa_K1896008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 05:52:47

INP_RC

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1896008_sequence (Version 1)

Description

INP_RC is a truncated variant of the inaZ from Pseudomonas syringae ([[Part:BBa_K1896001|Part:BBa_K1896001]]), the N-terminal domain was removed in order to prevent localisation to the outer membrane, while still maintaining ice nucleation activity. No stop codon is present in this part to allow seamless assembly of fusion proteins.

Notes

The repeat domain in this part seems to be impossible to create synthetically so PCR was used instead, with primers binding just outside of the repeat region. The absence of a stop codon allows for seamless assembly of fusion proteins.

Source

[[Part:BBa_K1896001|Part:BBa_K1896001]]

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
inaZ_RC	1,3096	feature/cds, CDS
start	1,3	start_codon, feature/start
repeat region	22,2949	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896008/1

Member of these Collections

• iGEM Parts Registry