Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ang Shi Hui
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 11:16:42
LuxR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1897008_sequence (Version 1) |
Description
This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015.This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI. This also produces light due to the production of LuxCDABE. Transcription of operonL leads to the production of LuxR. In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon.
This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). It also consists of an enzyme cut site XbaI after the biobricks prefix and a KpnI site before the biobricks suffix.
Notes
There is also one HA tag available for characterisation of the protein produced via western blot.Source
This part was made based on the genome of Vibrio fischeri.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0030 BBa_C0062 HA tag Stop Codon rrnBT1 BBa_B0012 | 1,35 36,50 63,812 813,839 840,842 843,914 923,963 | feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick sequence_feature feature/misc stop_codon feature/stop feature/misc sequence_feature feature/BioBrick engineered_region |