BBa_K1897011

BBa_K1897011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1897011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ang Shi Hui
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 12:54:39

Invasin



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1897011_sequence (Version 1)

Description

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, Invasin coding sequence BBa_K1897010 and double terminator BBa_B0015.

Notes

There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS BBa_K1897010 is shifted to after the HA tag.

Source

Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see BBa K1897010. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

Sequence Annotation Location Component / Role(s)
BBa_R0062
BBa_B0030
BBa_K1897010
HA tag
HA tag
Stop Codon
BBa_B0015
13,67
68,82
91,3048
3049,3075
3076,3102
3103,3105
3106,3234
engineered_region feature/BioBrick
engineered_region feature/BioBrick
engineered_region feature/BioBrick
sequence_feature feature/misc
feature/misc sequence_feature
feature/stop stop_codon
engineered_region feature/BioBrick
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1897011/1