BBa_K1897013

BBa_K1897013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1897013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ang Shi Hui

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 01:16:32

Listeriolysin O

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1897013_sequence (Version 1)

Description

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, Listeriolysin O (LLO) coding sequence BBa_K1897012 and double terminator BBa_B0015.

Notes

There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS BBa_K1897012 is shifted to after the HA tag.

Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is _______.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	21,75	feature/BioBrick, engineered_region
BBa_B0030	76,90	feature/BioBrick, engineered_region
BBa_K1897012	103,1689	feature/BioBrick, engineered_region
rrnBT1	1720,1791	feature/misc, sequence_feature
BBa_B0012	1800,1840	engineered_region, feature/BioBrick
HA tag	1690,1716	feature/misc, sequence_feature
Stop Codon	1717,1719	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1897013/1

Member of these Collections

• iGEM Parts Registry