BBa_K1897014

BBa_K1897014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1897014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ang Shi Hui

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 01:28:33

GFP with pLuxR

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1897014_sequence (Version 1)

Description

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, GFP coding sequence BBa_E0040 and double terminator BBa_B0015.

Notes

The transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.

Source

GFP was obtained from biobrick part BBa_E0040, which was derived from Aequeora victoria. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	15,69	feature/BioBrick, engineered_region
BBa_B0030	70,84	feature/BioBrick, engineered_region
BBa_E0040	97,813	engineered_region, feature/BioBrick
rrnBT1	814,885	feature/misc, sequence_feature
Stop Codon	811,813	stop_codon, feature/stop
BBa_B0012	894,934	engineered_region, feature/BioBrick

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1897014/1

Member of these Collections

• iGEM Parts Registry