Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM2016_HKUST
Date created: 2016-08-27 11:00:00
Date modified: 2016-10-15 04:03:09
Superfolder GFP
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1899003_sequence (Version 1) |
Description
Variant of GFP that folds robustly even when fused to poorly folded proteins.Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).