BBa_K190027

BBa_K190027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K190027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wilfred Poppinga

Date created: 2009-08-25 11:00:00

Date modified: 2015-05-08 01:11:15

MBP-ArsR fusion protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K190027_sequence (Version 1)

Description

A fusion of the Maltose binding protein and the ArsR repressor.

Notes

Adding tobacco etch virus (TEV) cleavage site and linker site to facilitate cloning. Adding a SacI restriction site to facilitate non-expensive cloning.

Source

ArsR was obtained from the E. coli K-12 DH10B genome
MBP was obtained from a plasmid

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
MBP	1,1100	feature/protein, CDS
start	1,3	feature/start, start_codon
TEV cleavage site	1101,1121	sequence_feature, feature/misc
ArsR	1134,1484	feature/protein, CDS
His10	1485,1515	feature/tag, tag
stop	1516,1518	stop_codon, feature/stop

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K190027/1

Member of these Collections

• iGEM Parts Registry