Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rafael Montenegro Mar??n
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-17 07:05:41
dCas9 B coding sequence
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1903017_sequence (Version 1) |
Description
The sequence is a coding part for a dCAs9 protein assembly with golden gate and has two windows frames for protein insertions at amino acids: N588 and N888Notes
This CDS was assembled with Golden Gate using four gBlocks.Source
The source for the Cas9 sequence was UNIPROT accession: Q99ZW2. We optimized the sequence for E. coli and changed the amino acids at positions D10A and H840A for making the protein catalytically inactive.| Sequence Annotation | Location | Component / Role(s) |
| dCas9 B version | 1,4134 | sequence_feature feature/misc |