BBa_K1904001

BBa_K1904001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1904001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Roger Milton Rubio S??nchez, Enrique Chimal, Enrique Palomino, Brian Morteo

Date created: 2016-06-05 11:00:00

Date modified: 2016-06-06 10:08:30

Endolysin with RBS and terminator. N-terminal HisTag

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K1904001_sequence (Version 1)

Description

This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with B0034 (RBS) and B0015 (double terminator), all in RFC10 standard. This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.

Notes

This biobrick was optimised for its usage in RFC10 standard and in E. coli.

Source

Acinetobacter baumanni phage PhIAB2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0034	1,12	feature/BioBrick, engineered_region
HisTag	22,39	feature/tag, tag
BBa_K1904000	19,594	CDS, feature/cds
B0015	595,723	engineered_region, feature/BioBrick
scar	13,18	feature/scar

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1904001/1

Member of these Collections

• iGEM Parts Registry