Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thomas Mathijs Swartjes
Date created: 2016-09-30 11:00:00
Date modified: 2016-10-19 04:53:17
434- and lambda cI operon for tuning protein balance
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K1913007_sequence (Version 1) |
Description
This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI protein encoded in this part contains a C-terminal LVA tag causing more rapid protein degradation, the lambda cI gene in this part does not encode an LVA tag.This part is intended to provide a balance between the 434- and lambda cI proteins depending on the strength of the RBS that is inserted upstream of 434 cI.
Notes
The LVA tag encoded by the lambda cI gene was purposefully left out of this part to provide different turnover rates for both cI proteins. This allow regulating the balance between the proteins based on changes in promoter strength over time.Source
This part was Gibson assembled from fragment originating from biobrick parts BBa_I0500, BBa_C0052, and BBa_K081007.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 pBAD BBa_C0052 BBa_K081007 no LVA 434 cI repressor lambda cI repressor | 1,1210 1080,1209 1211,1904 1905,2642 1211,1904 1926,2642 | engineered_region feature/BioBrick promoter feature/promoter feature/BioBrick engineered_region engineered_region feature/BioBrick CDS feature/cds feature/cds CDS |