BBa_K1913020

BBa_K1913020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1913020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tianhe Wang

Date created: 2016-10-06 11:00:00

Date modified: 2016-10-07 07:20:00

mRFP with degradation tag

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1913020_sequence (Version 1)

Description

mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

Notes

Making a mRFP coding sequence with fast degradation tag, which is easy for people to use it.

Source

iGEM part registry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0034	1,12	ribosome_entry_site, feature/rbs
E1010	19,693	feature/cds, CDS
6xHis tag	694,711	tag, feature/tag
M0050	717,756	tag, feature/tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1913020/1

Member of these Collections

• iGEM Parts Registry