BBa_K1913021

BBa_K1913021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1913021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 08:14:10

sfGFP with degradation tag



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K1913021_sequence (Version 1)

    Description

    sGFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

    Notes

    Making a sGPF coding sequence with degradation tag, which is convenient for people to build new composite part

    Source

    iGEM part registry

    Sequence Annotation Location Component / Role(s)
    B0034
    sfGFP
    M0050
    		1,12
    19,735
    736,774
    		feature/rbs ribosome_entry_site
    CDS feature/cds
    tag feature/tag

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1913021/1
     

    Attachments

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