BBa_K1915000

BBa_K1915000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1915000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Richardson
Date created: 2016-10-04 11:00:00
Date modified: 2016-10-17 06:38:55

LTNF10



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1915000_sequence (Version 1)

Description

LTNF10 (Lethal Toxin Neutralizing Factor) is a 10 residue peptide that has been shown to neutralize toxins from snakes, scorpions, and bacteria. LTNF peptides were first isolated from opossum (Didephis virginiana) serum. This part contains the LTNF10 peptide as well as C-term Myc and 6xHis tags that can be used for purification and then later removed using the HRV (Human Rhinovirus) protease.

Notes

N/A

Source

The sequence for the LTNF peptides were obtained from (G Chavan, S., & D Deobagkar, D. (2014). In Silico Molecular Interaction Analysis of LTNF Peptide-LT10 with Snake Venom Enzymes. Protein and peptide letters, 21(7), 646-656.)

The full sequence was synthesized by IDT.

Sequence Annotation Location Component / Role(s)
Start codon
LTNF10
HRV protease recognition site
Myc
6x His
Stop codon
1,3
4,34
35,58
59,89
104,121
120,123
feature/start start_codon
feature/protein CDS
feature/binding non_covalent_binding_site
tag feature/tag
feature/tag tag
stop_codon feature/stop
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1915000/1