Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 02:15:48
Ga0039498_104087g2 + intein-CBD tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1916007_sequence (Version 1) |
Description
A GAF domain of the cyanobacteriochrome (CBCR) Ga0039498_104087 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.Source
Cyanobacteria genome| Sequence Annotation | Location | Component / Role(s) |
| Ga0039498_104087g2 intein/chitin binding domain | 1,582 583,1365 | feature/protein CDS CDS feature/protein |