BBa_K1916007

BBa_K1916007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 02:15:48

Ga0039498_104087g2 + intein-CBD tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1916007_sequence (Version 1)

Description

A GAF domain of the cyanobacteriochrome (CBCR) Ga0039498_104087 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Cyanobacteria genome

Sequence Annotation Location Component / Role(s)
Ga0039498_104087g2
intein/chitin binding domain
1,582
583,1365
feature/protein CDS
CDS feature/protein
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916007/1