BBa_K1916021

BBa_K1916021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 12:26:22

CBCR9 + intein-CBD tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1916021_sequence (Version 1)

Description

Fusion protein consisting of the GAF domain of an uncharacterized cyanobacteriochrome (CBCR) sequence and an intein/chitin-binding domain attached to the C-terminus for purification. Labelled as CBCR9. RFC10 compatible.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Metagenomic data mining (JGI)

Sequence Annotation Location Component / Role(s)
CBCR9
intein/chitin binding domain
1,516
517,1299
feature/protein CDS
CDS feature/protein
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916021/1