BBa_K1916104

BBa_K1916104 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916104
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 11:44:39

Ara-Inducible Ga0100955_11928g3 Expression System



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1916104_sequence (Version 1)

Description

The Ga0100955_11928g3 protein (see BBa_K1916001) under control of a strong pBAD promoter.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

Sequence Annotation Location Component / Role(s)
BBa_K206000
conserved
BBa_B0034
Ga0100955_11928g3
intein/chitin binding domain
BBa_K1916004
1,130
143,146
139,150
157,750
751,1533
157,1533
feature/BioBrick engineered_region
conserved_region feature/conserved
feature/BioBrick engineered_region
CDS feature/protein
feature/protein CDS
feature/BioBrick engineered_region
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916104/1