BBa_K1916107

BBa_K1916107 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916107
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 11:22:05

Ara-Inducible Ga0039498_104087g2 Expression System



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1916107_sequence (Version 1)

Description

The Ga0039498_104087g2 protein (see BBa_K1916007) under control of a strong pBAD promoter. RFC10 compatible.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

Sequence Annotation Location Component / Role(s)
BBa_K206000
conserved
BBa_B0034
Ga0039498_104087g2
intein/chitin binding domain
BBa_K1916007
1,130
143,146
139,150
157,738
739,1521
157,1521
engineered_region feature/BioBrick
feature/conserved conserved_region
feature/BioBrick engineered_region
CDS feature/protein
feature/protein CDS
feature/BioBrick engineered_region
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916107/1