Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 01:02:38
Ara-Inducible CBCR4 Expression System
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1916115_sequence (Version 1) |
Description
The CBCR4 protein (see BBa_K1916015) under control of a strong pBAD promoter. RFC10 compatible.Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.Source
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.| Sequence Annotation | Location | Component / Role(s) |
| promoter AraI1 AraI2 BBa_K206000 conserved BBa_B0034 CBCR4 intein/chitin binding domain BBa_K1916015 | 1,131 40,57 61,78 1,130 143,146 139,150 157,657 658,1440 157,1440 | feature/promoter promoter feature/operator operator feature/operator operator feature/BioBrick engineered_region feature/conserved conserved_region feature/BioBrick engineered_region CDS feature/protein CDS feature/protein feature/BioBrick engineered_region |