Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoyu Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 02:22:20
J23114 promoter-Constitutive AarC Expression Device
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1919214_sequence (Version 1) |
Description
This device can express AarC with His-tag. Promoter is BBa_J23114. His-tag and T7 terminator come from pET-23 vector.Notes
The AarC conding sequence with His-tag and T7 terminator were cloned from pET-23-AarC express vector. Promoter and RBS were added by PCR.Source
The coding sequence of AarC comes from BBa_K1919233.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23114 His tag BBa_B0034 ATG start codon AarC coding secquence double TAA stop codon T7 terminator | 1,35 1584,1601 44,56 63,65 63,1582 1616,1618 1669,1714 | feature/BioBrick engineered_region tag feature/tag engineered_region feature/BioBrick feature/start start_codon feature/cds CDS feature/stop stop_codon feature/misc sequence_feature |