Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoyu Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 02:09:50
AarC coding sequence
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1919233_sequence (Version 1) |
Description
Succinyl-CoA:Acetate CoA-Transferase(AarC) can let acetate and Succinyl-CoA become acetyl-CoA. E.coli can use acetate as energy sources by this enzyme. This enzyme also can give E.coli some ability of anti acetic acid.Notes
The coding sequence was optimized for E.coli express by Genscript. A restriction enzyme cleavage site of PstI in gene was mutated by oe-pcr.Source
The original sequence of AarC comes from GenBank NC_021991.1. The sample used for experiments was artificially systhesized by Synbio Technologies Co., Ltd.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon AarC coding secquence double TAA stop codon | 1,3 1,1521 1516,1521 | start_codon feature/start feature/cds CDS stop_codon feature/stop |