BBa_K1919300

BBa_K1919300 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1919300

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shaobo Yang

Date created: 2016-09-15 11:00:00

Date modified: 2016-10-21 12:04:00

livJ

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1919300_sequence (Version 1)

Description

This part contains a promoter, RBS, protein coading region and a terminator. The expression of the protein livJ in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.

LivJ is a crutial subunit of leucine transporter which is regulated by xxx. Overexpression of livJ can increase the untilizaiton of leucine in E.coli.

Notes

update later

Source

LivJ was cloned from genome of E.coli DH5a. Please note that there is no such gene in genome of E.coli BL21

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23100	1,35	feature/BioBrick, engineered_region
B0030	44,58	engineered_region, feature/BioBrick
terminator B0010	1169,1248	engineered_region, feature/BioBrick
livJ coding region	65,1168	feature/BioBrick, engineered_region

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1919300/1

Member of these Collections

• iGEM Parts Registry