Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyang Mao
Date created: 2016-10-23 11:00:00
Date modified: 2016-10-24 10:06:13
Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1923011_sequence (Version 1) |
Description
This composite part is a fusion protein composed of GAL4, triple FLAG tag, two SV40 nuclear localization sequence,dCas9 and GFP. Triple FLAG tag and GFP is designed for observation and expression detection. The protein produced by this parts can be sequestered in the cytoplasm with the help of sgRNA and PAMmer, utilizing dCas9's mRNA binding ability. However, when there is a mutation in the target sequence of the mRNA, sv40 NLS would drag this protein into the nuclear. Then GAL4 would active the gene downstream UAS promoter. Thus this parts can be used for gene mutation surveillance [1].Notes
This part is listed for the gold medal criterion (Improve the function OR characterization of a previously existing BioBrick Part or Device).Source
This part was assembled from previous BioBricks (BBa_K1323002, BBa_K1493504 and BBa_K1470002) originating from distribution kits, and was integrated into a fusion protein.| Sequence Annotation | Location | Component / Role(s) |
| Gal4 binding domain Nucleus localization sequence Triple FLAG tag EGFP encoding gene dCas9 encoding gene Nucleus localization sequence Gal4 activating domain | 3,444 481,502 503,569 576,1295 1296,5400 5401,5422 5445,5796 | CDS feature/cds feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds feature/cds CDS CDS feature/cds |