Types | DnaRegion
|
Roles | engineered_region
Project
|
Sequences | BBa_K1926001_sequence (Version 1)
|
Description
This part is a cyclic promoter named Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]
1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.
2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.
Notes
None
Source
The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
pCCNE-R: TGATGGGGCTGCTCCGGCCT
(Product: 986)