Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyi Liu
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-11 05:50:50
The mCHERRY UNIT: mCherry flanked by Vox
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1926013_sequence (Version 1) |
Description
This part is the mCHERRY UNIT of cyclebow system including a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA.You may use this part to:
1) Provide fluorescent red which can be quickly cut off by transient transfecting recombinase gene into nucleus;
2) Use it as a part of the flp-out system[5].
5. Voutev, R. and E.J. Hubbard, A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Genetics, 2008. 180(1): p. 103-19.
Notes
NoneSource
The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY and sv40 terminator were got from commercial plasmid: pentry dcas9 mcherry bira and pentry nls GFP/pcdna3.1, respectfully.| Sequence Annotation | Location | Component / Role(s) |
| vox mCherry mutate C into A to remove Pst1 sv40 terminator vox TAA | 1,34 35,739 384,384 740,882 883,916 759,761 | feature/binding non_covalent_binding_site feature/misc sequence_feature feature/mutation sequence_alteration sequence_feature feature/dna feature/binding non_covalent_binding_site stop_codon feature/stop |