Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Barbora Waclawikova
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-17 09:01:43
Encrypted message sequence in integration plasmid (BBa_K823023)
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1930003_sequence (Version 1) |
Description
The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.Notes
The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/ExperimentsSource
Message sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023| Sequence Annotation | Location | Component / Role(s) |
| BBa_K823023 message sequence cat amyE bla amyE BBa_K1930001 | 557,6618 1,556 893,1543 1914,2942 4896,5756 6081,6601 1,556 | engineered_region feature/BioBrick sequence_feature feature/dna feature/cds CDS feature/misc sequence_feature feature/cds CDS sequence_feature feature/misc engineered_region feature/BioBrick |