| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1933011_sequence (Version 1)
|
Description
INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein (INP) from Pseudomonas syringae , connected by a 6xHis tag to be easily identified by Western blotting[1]. This is used as an anchoring motif for the cell surface display of passengers. This domain does not have signal peptide for anchoring on the cell surface[2]. Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6xHis tag.
Notes
We omitted stop codon from BBa_K1933001 to use it for the registration of composite parts.
Source
INPNC derived from BBa_K811005
