Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 01:18:16
pNagA-RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1937003_sequence (Version 1) |
Description
The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate.
This NAG molecule is the major component of chitin, a characteristic molecule of fungi.
Notes
The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.Source
D'o?? vient le promoteur, le RBS, la s??quence codante...la s??quence en tant que telle aussi
| Sequence Annotation | Location | Component / Role(s) |
| Promoter NagP+RBS RFP Terminator | 1,200 207,912 913,992 | feature/promoter promoter CDS feature/cds stop_codon feature/stop |