BBa_K1937003

BBa_K1937003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1937003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 01:18:16

pNagA-RFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1937003_sequence (Version 1)

Description

The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate.
This NAG molecule is the major component of chitin, a characteristic molecule of fungi.

Notes

The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.

Source

D'o?? vient le promoteur, le RBS, la s??quence codante...
la s??quence en tant que telle aussi

Sequence Annotation Location Component / Role(s)
Promoter NagP+RBS
RFP
Terminator
1,200
207,912
913,992
feature/promoter promoter
CDS feature/cds
stop_codon feature/stop
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1937003/1