BBa_K1939000

BBa_K1939000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1939000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyler Herrington
Date created: 2016-09-28 11:00:00
Date modified: 2016-09-29 07:21:42

Mutant RBS with mCherry reporter gene



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1939000_sequence (Version 1)

Description

This composite part contains an RBS with a single point mutation (C to G transversion) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in higher translational efficiency with mCherry fluorescence that is 116.88% of the native RBS.

Notes

This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers.

Source

This is a mutant of the RBS region of the native strain, S001, of M. maripaludis

Sequence Annotation Location Component / Role(s)
Promoter
Mutant RBS
mCherry
BBa_K1383000
2,175
185,190
196,906
1,906
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/protein CDS
engineered_region feature/BioBrick
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1939000/1