BBa_K1939001

BBa_K1939001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1939001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyler Herrington
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 02:49:15

Mutant RBS with mCherry reporter gene



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1939001_sequence (Version 1)

Description

This composite part contains an RBS with a single point mutation (G to A transition) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in higher translational efficiency with mCherry fluorescence that is 165.85% of the native RBS.

Notes

This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers.

Source

This is a mutant of the RBS region of the native strain, S001, of M. maripaludis

Sequence Annotation Location Component / Role(s)
Promoter
Native RBS
mCherry
BBa_K1383000
2,175
185,190
196,906
1,906
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/protein
feature/BioBrick engineered_region
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1939001/1