BBa_K194002

BBa_K194002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K194002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christian Kaas
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:16

Destabilized GFP for yeast



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K194002_sequence (Version 1)

Description

This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000.
In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

Notes

follows standard biobrick standards

Source

Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry"

Sequence Annotation Location Component / Role(s)
yeast GFP CDS
cln2 PEST domain CDS
1,707
708,1257
CDS feature/cds
feature/cds CDS
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BBa_K194002/1