Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K194002_sequence (Version 1)
|
Description
This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000.
In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.
Notes
follows standard biobrick standards
Source
Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry"