Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shixin Lu, Shiqiang Tang
Date created: 2016-08-28 11:00:00
Date modified: 2016-10-21 08:00:08
fwYellow, yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1943000_sequence (Version 1) |
Description
Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943000 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization.We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
Notes
11Source
basic parts| Access | Instance | Definition |
| public public public | BBa_K880005 BBa_K1033910 BBa_B0015 | BBa_K880005 fwYellow BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K880005 BBa_K1033910 BBa_B0015 | 1,55 62,775 784,912 | BBa_K880005 fwYellow BBa_B0015 |