BBa_K1943002

BBa_K1943002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1943002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shixin Lu, Shiqiang Tang
Date created: 2016-08-28 11:00:00
Date modified: 2016-10-21 08:03:17

gfasPurple, purple chromoprotein reporter system (Strong Promoter, Strong RBS)



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K1943002_sequence (Version 1)

Description

Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943002 with chromoprotein gfasPurple(BBa_K1033919) this year and did characterization.
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
For detailed characterization data, see the experience page.

Notes

no

Source

basic parts

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1943002/1