Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Musa Efe Işılak
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 05:53:57
Cycloxehanone Sensor
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1946003_sequence (Version 1) |
Description
This part consists codon optimised ChnR (BBa_K1946000) from acinetobacter sp. operated by Tet operator and promoter region of ChnB (BBa_K1946001) followed by sfGFP (BBa_I746916). It is shown that ChnR activates ChnB protein in the presence of cyclohexanone. This sensor generates sfGFP in the presence of cyclohexanone.Notes
ChnR gene synthesised with respect to Uniprot amino acid sequence. Codon optimised for E. Coli k12Source
ChnR protein and pChnB promoter from acinetobacter sp.| Sequence Annotation | Location | Component / Role(s) |
| pL(tetO) rbs BBa_K1946000 B0010 BBa_K1946001 rbs BBa_I746916 B0010 | 1,56 57,78 79,1020 1021,1125 1132,1668 1675,1689 1702,2415 2425,2529 | promoter feature/promoter ribosome_entry_site feature/rbs feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/BioBrick engineered_region |