BBa_K1946007

BBa_K1946007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1946007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Musa Efe Işılak
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 04:40:15

dCas9 E. Coli



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1946007_sequence (Version 1)

    Description

    dCas9 is a catalytically inactive Cas9 that cannot generate double strand cleavage. It recognises the sequence targeted by gRNA and binds to that sequence. It can be used for gene silencing and in an AND gate. Further information can be found here: http://2016.igem.org/Team:Bilkent-UNAMBG/Description

    Notes

    The part was codon optimized for E. Coli k12.

    Source

    This dCas9 was ordered from Addgene (ID:44249).

    Sequence Annotation Location Component / Role(s)
    dCas9
    start
    stop
    		1,4107
    1,3
    4105,4107
    		CDS feature/cds
    start_codon feature/start
    feature/stop stop_codon

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1946007/1
     

    Attachments

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