Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: claire raynaud
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 07:28:58
DesA producer under PBAD/araC promotor
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1951004_sequence (Version 1) |
Description
This coding sequence is a producer of Lysine descarboxylase (Streptomyces coelicolor) which is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
The production of the protein has been controled by a PBAD promoter inductible with arabinose (0.01% arabinose added in the culture medium. (ref : Bba10500)
The RBS used is the Bba B0034.
Notes
There is no special design consideration for this part.Source
PBAD : Bba10500RBS : BbaB0034
desA sequence :
On the following link, please find the gene reference and its chromosomal location. http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=map
Start : 3035641
End : 3037083
Coding sequence of desA comes from the website below :
http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
| Sequence Annotation | Location | Component / Role(s) |
| pBAD/araC (BbaI0500) BbaB0034 DesA (BbaK1951000) Double stop | 1,30 31,40 41,1488 1484,1488 | feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein stop_codon feature/stop |