BBa_K1951008

BBa_K1951008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1951008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: claire raynaud
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 01:52:13

FliC E.coli producer



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K1951008_sequence (Version 1)

Description

Flagellin C (FliC) protein from Escherichia coli strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as Platinum, gold...
We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110.

Notes

On the BbaK1951004, the restriction site Bbs1 is mutated (substitution) to allow the control for a future Bbs1 site insertion.

Source

We made this part from 2 others :
- BbaK1951004
- Bba880005

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1951008/1