Types | DnaRegion
|
Roles | sequence_feature
DNA
|
Sequences | BBa_K1951008_sequence (Version 1)
|
Description
Flagellin C (FliC) protein from Escherichia coli strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as Platinum, gold...
We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110.
Notes
On the BbaK1951004, the restriction site Bbs1 is mutated (substitution) to allow the control for a future Bbs1 site insertion.
Source
We made this part from 2 others :
- BbaK1951004
- Bba880005