Types | DnaRegion
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Roles | engineered_region
Composite
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Sequences | BBa_K1955009_sequence (Version 1)
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Description
In order to prove the OVA sequence which we designed can smoothly express protein, we use BL21 competent cell to express the OVA sequence and detect the protein by Western blot analysis.
Notes
Use the standard parts provided by iGEM, we choose BBa_J04500 as a promoter to induce production of protein. BBa_J04500 is a LacI inducible promoter with RBS, so it can be induced by IPTG to open its promoter and start transcription. Double restriction enzyme cutting(EcoRI and XbaI) of pSB1C3-HA to produce a sticky end in the anterior, then also double restriction enzyme cutting(EcoRI and SpeI) of BBa_J04500 to produce other sticky end which can be ligated with EcoRI and XbaI sticky end. Moreover, it will form a scar when XbaI ligate with SpeI, so we can simply compose the pSB1C3-J04500-OVA.
Source
LacI inducible promoter came from BBa_J04500; OVA came from BBa_K1955004.