Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu-chang Ku, Pang-ling Huang
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 06:08:08
pSB1A2-LacI inducible GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1955010_sequence (Version 1) |
Description
We will use BBa_E0040, a GFP generator, as a positive control to confirm that our process in production of protein has no problem.Notes
Use the standard parts provided by iGEM, we choose BBa_J04500 as a promoter to induce production of protein. BBa_J04500 is a LacI inducible promoter with RBS, so it can be induced by IPTG to open its promoter and start transcription. Double restriction enzyme cutting(EcoRI and XbaI) of pSB1A2-GFP to produce a sticky end in the anterior, then also double restriction enzyme cutting(EcoRI and SpeI) of BBa_J04500 to produce other sticky end which can be ligated with EcoRI and XbaI sticky end. Moreover, it will form a scar when XbaI ligate with SpeI, so we can simply compose the pSB1A2-J04500-GFP.Source
LacI inducible promoter came from BBa_J04500; GFP protein sequence came from BBa_E0040.| Access | Instance | Definition |
| public public | BBa_J04500 BBa_E0040 | BBa_J04500 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_E0040 | 1,220 227,946 | BBa_J04500 GFP |