Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Wei
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 08:59:59
HyaA -> E. coli
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1958001_sequence (Version 1) |
Description
HyaA is the small subunit of hydrogenase 1 (Hyd-1, from MBH family) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structural subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaA is the first gene of hya operon, sharing the last few base pairs with hyaB. Under carbon starvation upon entering stationary phase, expression of the hya operon, which encodes Hyd-1, is regulated by sigma-38. The small subunit exhibits sequence homologies to subunits of NADH: ubiquinone oxidoreductase (Complex I). This small subunit of this [Ni-Fe] hydrogenase contains 4 Fe-S clusters ([Fe3S4], [Fe4S4]d, [Fe4S3]) that play a role in ET to and from the large subunit for H2 reduction/oxidation. The supernumerary cysteine in the proximal cluster of the small subunit also plays a role in O2 tolerance.Notes
NoneSource
Escherichia coli BL21| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TGA stop codon HyaA coding region | 1,3 1117,1119 1,1119 | feature/start start_codon feature/stop stop_codon feature/cds CDS |