Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junyu Chen
Date created: 2016-10-02 11:00:00
Date modified: 2016-10-24 02:00:18
Modification of phytoene desaturase (PDS/CrtI)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1959001_sequence (Version 1) |
Description
This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. Codon optimized has been made for the expression inOrazy sativa.
Notes
The sequence has been optimized for expression in Orazy sativa and two restriction sites have been mutated in order to fit the requirement of the Registry.Source
The original sequence of this part can be accessed from Genebank. Genebank accession:D90087.| Sequence Annotation | Location | Component / Role(s) |
| CtrI coding sequence Pea transit peptide Initiation codon of transit peptide Initiation codon of Crt I Termination codon Pst I mutation Pst I mutation | 172,1650 1,171 1,3 172,174 1648,1650 1060,1065 1619,1624 | CDS feature/cds CDS feature/cds start_codon feature/start feature/start start_codon feature/stop stop_codon feature/s_mutation point_mutation feature/s_mutation point_mutation |