Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junyu Chen
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-19 12:18:07
Modification of β
-carotene hydroxylase (BHY)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1959002_sequence (Version 1) |
Description
This part codes for beta-carotene hydroxylase, which is needed for astaxanthin biosynthesis. It catalyzes the convertion of beta-carotene to zeaxanthin. The part is original from Haematococcus pluvialis and codon optimization has been made in order to efficientlyexpress in Orazy sativa.
Notes
Codon optimization has been made for expression in Orazy sativa and a PstI restriction site has mutated.Source
The origin BHY sequence can be accessed from Genebank. Genebank accession: BD250390.1 .| Sequence Annotation | Location | Component / Role(s) |
| Pst I mutation BHY cds termination codon Pea transit peptide Initiation codon of Pea transit peptide Initiation codon of BHY cds | 352,357 172,1053 1051,1053 1,171 1,3 172,174 | feature/s_mutation point_mutation CDS feature/cds stop_codon feature/stop CDS feature/cds feature/start start_codon start_codon feature/start |